Cell transfer and PCR
Biopsied cells are washed then transferred into a PCR reaction tube using a fine glass pipette. DNA is extracted from the cells and a PCR reaction mix added. The tubes are placed in a PCR machine where DNA is amplified from a few copies to millions.
Linkage Marker and Mutation Testing
Single Gene Disorders:
a) Family Linkage – Recessive Disorders
A recessive disorder requires a copy of the affected gene from each parent for an embryo to be affected
b) Family Linkage – Dominant Disorders
A dominant disorder requires one copy of the affected gene, inherited from an affected parent, for an embryo to be affected
An embryo must inherit a complete set of chromosomes in order to be suitable for transfer
Aneuploidy / Sex Selection
The status for chromosomes X, Y and 21 of an embryo can be determined by looking at the DNA inheritance from both parents.